antibodies egfl7  (Proteintech)

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: Primer sequences used in PCR.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques:

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: TMP promotes angiogenesis, nerve regeneration and nerve defect repair in rats with SCI. A: BBB score to test the motor behavior of rats; B–C: HE staining and TUNEL staining to assess the lesions of rat spinal cord; D: Western blot to detect CD31 expression to assess angiogenesis; E–G: ELISA to detect inflammatory factors; H–I: RT-qPCR or Western blot to detect miR-497-5p and EGFL7 expression; the values are expressed by the mean standard deviation. * P < 0.05 vs. Sham; # P < 0.05 vs. Model.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Staining, TUNEL Assay, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Standard Deviation

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: MiR-497-5p inhibits EGFL7 expression. A: Bioinformatics website to predict that miR-497-5p and EGFL7 have targeted binding sites; B: Dual-luciferase reporter gene detection to confirm the targeting relationship between miR-497-5p and EGFL7; C –D: RT-qPCR and Western blot to detect EGFL7 expression; the values were expressed by the mean standard deviation. * P < 0.05 vs. TMP + antagomir NC; # P < 0.05 vs. TMP + agomir NC.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Expressing, Binding Assay, Luciferase, Quantitative RT-PCR, Western Blot, Standard Deviation

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: EGFL7 upregulation promotes TMP-mediated protection on SCI rats. A: RT-qPCR or Western blot to detect EGFL7 expression; B: BBB score to test the motor behavior of rats; C –D: HE staining and TUNEL staining to assess the lesions of the rat spinal cord; E: Western blot to detect CD31 expression to assess angiogenesis; F–H: ELISA detects the levels of inflammatory factors; the values are expressed by the mean standard deviation. * P < 0.05 vs. TMP + oe-NC; # P < 0.05 vs. TMP + sh-NC.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: EGFL7 inhibition resists miR-497-5p silencing-induced protection against SCI. A: RT-qPCR or Western blot to detect EGFL7 expression; B: BBB score to test the motor behavior of rats; C –D: HE staining and TUNEL staining to assess the lesions of the rat spinal cord; E: Western blot to detect CD31 expression to assess angiogenesis; F–H: ELISA detects the levels of inflammatory factors; the values are expressed by the mean standard deviation. * P < 0.05 vs. TMP + miR-497-5p antagomir + sh-NC.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Inhibition, Quantitative RT-PCR, Western Blot, Expressing, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation